Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA)
- PMID: 16179424
- DOI: 10.1373/clinchem.2005.051532
Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA)
Abstract
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully automated instruments in medical laboratories around the world use the immunoassay principle with an enzyme as the reporter label for routine measurements of innumerable analytes in patient samples. The impact of EIA/ELISA is reflected in the overwhelmingly large number of times it has appeared as a keyword in the literature since the 1970s. Clinicians and their patients, medical laboratories, in vitro diagnostics manufacturers, and worldwide healthcare systems owe much to these four inventors.
Comment in
-
Perspective on the historical note on EIA/ELISA by Dr. R.M. Lequin.Clin Chem. 2005 Dec;51(12):2225. doi: 10.1373/clinchem.2005.059618. Clin Chem. 2005. PMID: 16306091 No abstract available.
-
The rise of EIA/ELISA.Clin Chem. 2005 Dec;51(12):2226. doi: 10.1373/clinchem.2005.059626. Clin Chem. 2005. PMID: 16306092 No abstract available.
-
Historical background of the invention of EIA and ELISA.Clin Chem. 2006 Jul;52(7):1430-1; author reply 1431. doi: 10.1373/clinchem.2006.069724. Clin Chem. 2006. PMID: 16798968 No abstract available.
Similar articles
-
A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA.Peptides. 2015 Oct;72:4-15. doi: 10.1016/j.peptides.2015.04.012. Epub 2015 Apr 20. Peptides. 2015. PMID: 25908411
-
Historical background of the invention of EIA and ELISA.Clin Chem. 2006 Jul;52(7):1430-1; author reply 1431. doi: 10.1373/clinchem.2006.069724. Clin Chem. 2006. PMID: 16798968 No abstract available.
-
Perspective on the historical note on EIA/ELISA by Dr. R.M. Lequin.Clin Chem. 2005 Dec;51(12):2225. doi: 10.1373/clinchem.2005.059618. Clin Chem. 2005. PMID: 16306091 No abstract available.
-
Chapter 5. Potential contribution of sero-epidemiological analysis for monitoring malaria control and elimination: historical and current perspectives.Adv Parasitol. 2009;69:299-352. doi: 10.1016/S0065-308X(09)69005-9. Adv Parasitol. 2009. PMID: 19622411 Review.
-
European Union funded project on the development of a whole complement deficiency screening ELISA-A story of success and an exceptional manager: Mohamed R. Daha.Mol Immunol. 2015 Nov;68(1):63-6. doi: 10.1016/j.molimm.2015.05.003. Epub 2015 May 23. Mol Immunol. 2015. PMID: 26006049 Review.
Cited by
-
Externally sensitized deprotection of PPG-masked carbonyls as a spatial proximity probe in photoamplified detection of binding events.Photochem Photobiol Sci. 2012 Mar;11(3):564-77. doi: 10.1039/c2pp05326h. Epub 2012 Jan 18. Photochem Photobiol Sci. 2012. PMID: 22252455 Free PMC article.
-
Potential role of tea drinking in preventing hyperuricaemia in rats: biochemical and molecular evidence.Chin Med. 2022 Sep 15;17(1):108. doi: 10.1186/s13020-022-00664-x. Chin Med. 2022. PMID: 36109783 Free PMC article.
-
A novel colorimetric immunoassay utilizing the peroxidase mimicking activity of magnetic nanoparticles.Int J Mol Sci. 2013 May 10;14(5):9999-10014. doi: 10.3390/ijms14059999. Int J Mol Sci. 2013. PMID: 23665902 Free PMC article.
-
Non-Invasive Biomarkers for Early Lung Cancer Detection.Cancers (Basel). 2022 Nov 24;14(23):5782. doi: 10.3390/cancers14235782. Cancers (Basel). 2022. PMID: 36497263 Free PMC article. Review.
-
Molecular fingerprinting of nanoparticles in complex media with non-contact photoacoustics: beyond the light scattering limit.Sci Rep. 2018 Sep 26;8(1):14425. doi: 10.1038/s41598-018-32580-2. Sci Rep. 2018. PMID: 30258194 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
