Kinetics of biomarkers: biological and technical validity of isoprostanes in plasma
- PMID: 16049640
- DOI: 10.1007/s00726-005-0229-2
Kinetics of biomarkers: biological and technical validity of isoprostanes in plasma
Abstract
Isoprostanes, non-enzymatic peroxidation products of arachidonic acid, are attractive biomarkers of oxidative stress in research in biology, medicine and nutrition. For the appropriate use of biomarkers it is required that these are both biologically and technically valid. Whereas the biological validity of isoprostanes is well-established, it is technically quite complicated to measure isoprostanes and its metabolites in body fluids, and its rapid disappearance from plasma may hamper practical application. This paper shortly introduces isoprostanes as a biomarker for studies with humans, describes a novel fast and sensitive method for measuring isoprostanes in plasma by high-performance liquid chromatography and tandem mass spectrometry, and provides several examples of the use of the method in studies in humans. By taking care of the biological and technical validity of this biomarker it is possible to establish the antioxidant effects of some food ingredients in studies with human volunteers.
Similar articles
-
[Isoprostanes--new possibility of the oxidative stress estimation].Przegl Lek. 2004;61(12):1410-4. Przegl Lek. 2004. PMID: 15850339 Review. Polish.
-
Biomarkers of oxidative stress study III. Effects of the nonsteroidal anti-inflammatory agents indomethacin and meclofenamic acid on measurements of oxidative products of lipids in CCl4 poisoning.Free Radic Biol Med. 2005 Mar 15;38(6):711-8. doi: 10.1016/j.freeradbiomed.2004.10.024. Free Radic Biol Med. 2005. PMID: 15721981
-
[Biomarkers of lipid peroxidation: analytical aspects].Ann Biol Clin (Paris). 2008 Nov-Dec;66(6):605-20. doi: 10.1684/abc.2008.0283. Ann Biol Clin (Paris). 2008. PMID: 19091659 Review. French.
-
Quantification of F2-isoprostanes in biological fluids and tissues as a measure of oxidant stress.Methods Enzymol. 2007;433:113-26. doi: 10.1016/S0076-6879(07)33006-1. Methods Enzymol. 2007. PMID: 17954231
-
Fatty acid oxidation and isoprostanes: oxidative strain and oxidative stress.Prostaglandins Leukot Essent Fatty Acids. 2010 Apr-Jun;82(4-6):219-25. doi: 10.1016/j.plefa.2010.02.031. Epub 2010 Apr 2. Prostaglandins Leukot Essent Fatty Acids. 2010. PMID: 20363116 Review.
Cited by
-
Prevention of UVB Induced Metabolic Changes in Epidermal Cells by Lipid Extract from Microalgae Nannochloropsis oceanica.Int J Mol Sci. 2023 Jul 11;24(14):11302. doi: 10.3390/ijms241411302. Int J Mol Sci. 2023. PMID: 37511067 Free PMC article.
-
Diversified Effects of COVID-19 as a Consequence of the Differential Metabolism of Phospholipids and Lipid Peroxidation Evaluated in the Plasma of Survivors and Deceased Patients upon Admission to the Hospital.Int J Mol Sci. 2022 Oct 5;23(19):11810. doi: 10.3390/ijms231911810. Int J Mol Sci. 2022. PMID: 36233111 Free PMC article.
-
The Impact of Severe COVID-19 on Plasma Antioxidants.Molecules. 2022 Aug 21;27(16):5323. doi: 10.3390/molecules27165323. Molecules. 2022. PMID: 36014561 Free PMC article.
-
The Effect of β-Carotene, Tocopherols and Ascorbic Acid as Anti-Oxidant Molecules on Human and Animal In Vitro/In Vivo Studies: A Review of Research Design and Analytical Techniques Used.Biomolecules. 2022 Aug 7;12(8):1087. doi: 10.3390/biom12081087. Biomolecules. 2022. PMID: 36008981 Free PMC article. Review.
-
Metabolic Response to Tick-Borne Encephalitis Virus Infection and Bacterial Co-Infections.Pathogens. 2022 Mar 23;11(4):384. doi: 10.3390/pathogens11040384. Pathogens. 2022. PMID: 35456059 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
