c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I
- PMID: 15723054
- DOI: 10.1038/ncb1224
c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I
Erratum in
- Nat Cell Biol. 2005 May;7(5):531
Abstract
c-Myc coordinates cell growth and division through a transcriptional programme that involves both RNA polymerase (Pol) II- and Pol III-transcribed genes. Here, we demonstrate that human c-Myc also directly enhances Pol I transcription of ribosomal RNA (rRNA) genes. rRNA synthesis and accumulation occurs rapidly following activation of a conditional MYC-ER allele (coding for a Myc-oestrogen-receptor fusion protein), is resistant to inhibition of Pol II transcription and is markedly reduced by c-MYC RNA interference. Furthermore, by using combined immunofluorescence and rRNA-FISH, we have detected endogenous c-Myc in nucleoli at sites of active ribosomal DNA (rDNA) transcription. Our data also show that c-Myc binds to specific consensus elements located in human rDNA and associates with the Pol I-specific factor SL1. The presence of c-Myc at specific sites on rDNA coincides with the recruitment of SL1 to the rDNA promoter and with increased histone acetylation. We propose that stimulation of rRNA synthesis by c-Myc is a key pathway driving cell growth and tumorigenesis.
Comment in
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The Myc trilogy: lord of RNA polymerases.Nat Cell Biol. 2005 Mar;7(3):215-7. doi: 10.1038/ncb0305-215. Nat Cell Biol. 2005. PMID: 15738972 No abstract available.
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