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. 2004 Dec 29;119(7):941-53.
doi: 10.1016/j.cell.2004.12.012.

Histone demethylation mediated by the nuclear amine oxidase homolog LSD1

Yujiang Shi  1 , Fei Lan, Caitlin Matson, Peter Mulligan, Johnathan R Whetstine, Philip A Cole, Robert A Casero, Yang Shi
Affiliations
Free article

Histone demethylation mediated by the nuclear amine oxidase homolog LSD1

Yujiang Shi et al. Cell. .
Free article

Abstract

Posttranslational modifications of histone N-terminal tails impact chromatin structure and gene transcription. While the extent of histone acetylation is determined by both acetyltransferases and deacetylases, it has been unclear whether histone methylation is also regulated by enzymes with opposing activities. Here, we provide evidence that LSD1 (KIAA0601), a nuclear homolog of amine oxidases, functions as a histone demethylase and transcriptional corepressor. LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription. Lysine demethylation occurs via an oxidation reaction that generates formaldehyde. Importantly, RNAi inhibition of LSD1 causes an increase in H3 lysine 4 methylation and concomitant derepression of target genes, suggesting that LSD1 represses transcription via histone demethylation. The results thus identify a histone demethylase conserved from S. pombe to human and reveal dynamic regulation of histone methylation by both histone methylases and demethylases.

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Comment in

  • A crack in histone lysine methylation.
    Kubicek S, Jenuwein T. Kubicek S, et al. Cell. 2004 Dec 29;119(7):903-6. doi: 10.1016/j.cell.2004.12.006. Cell. 2004. PMID: 15620348 Review.
  • Taking LSD 1 to a new high.
    Wysocka J, Milne TA, Allis CD. Wysocka J, et al. Cell. 2005 Sep 9;122(5):654-8. doi: 10.1016/j.cell.2005.08.022. Cell. 2005. PMID: 16143099 Review.
  • All wrapped up in histones.
    David R. David R. Nat Rev Mol Cell Biol. 2010 Oct;11(10):680-1. doi: 10.1038/nrm2981. Nat Rev Mol Cell Biol. 2010. PMID: 20861877 No abstract available.

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