Effect of streptozotocin-induced diabetes on rat brain mitochondria
- PMID: 14962073
Effect of streptozotocin-induced diabetes on rat brain mitochondria
Abstract
This study evaluated several parameters related to mitochondrial function and oxidative stress in streptozotocin (STZ)-treated rats, a model of type 1 diabetes. For this purpose, the respiratory indexes (RCR and ADP/O ratio), mitochondrial transmembrane potential (DeltaPsim), repolarization lag phase, repolarization level, mitochondrial enzymatic activities, ATP and malondialdehyde (MDA) levels, reduced glutathione (GSH), vitamin E and cardiolipin contents were determined in rat brain mitochondria isolated after 4 and 9 weeks after STZ treatment. Brain mitochondria isolated from citrate (vehicle)-treated Wistar rats were used as control. We observed that STZ-induced diabetes did not substantially affect brain mitochondrial function. Instead, 4-week diabetic rats presented higher mitochondrial respiratory chain enzymatic activities, especially succinate-cytochrome C reductase activity, compared to 4-week control rats. In 9-week diabetic rats, only a significant decrease in cardiolipin content was observed; however, a significant increase in mitochondrial GSH content occurred. All other parameters analysed remained statistically unchanged. From these results, we conclude that STZ-induced diabetes did not promote brain mitochondrial dysfunction, suggesting that oxidative stress associated with type 1 diabetes is not directly related to mitochondrial dysfunction, but probably is related to extramitochondrial factor(s).
Similar articles
-
Insulin protects against amyloid beta-peptide toxicity in brain mitochondria of diabetic rats.Neurobiol Dis. 2005 Apr;18(3):628-37. doi: 10.1016/j.nbd.2004.10.017. Neurobiol Dis. 2005. PMID: 15755688
-
Insulin-induced recurrent hypoglycemia exacerbates diabetic brain mitochondrial dysfunction and oxidative imbalance.Neurobiol Dis. 2013 Jan;49:1-12. doi: 10.1016/j.nbd.2012.08.008. Epub 2012 Aug 24. Neurobiol Dis. 2013. PMID: 22940631
-
Attenuation of myocardial apoptosis by alpha-lipoic acid through suppression of mitochondrial oxidative stress to reduce diabetic cardiomyopathy.Chin Med J (Engl). 2009 Nov 5;122(21):2580-6. Chin Med J (Engl). 2009. PMID: 19951573
-
Effect of streptozotocin on glutathione and lipid peroxide levels in various tissues of rats.Res Commun Mol Pathol Pharmacol. 1998 Dec;102(3):265-72. Res Commun Mol Pathol Pharmacol. 1998. PMID: 10342913
-
[Brain mitochondria].Tanpakushitsu Kakusan Koso. 1966 Jan;11(1):60-8. Tanpakushitsu Kakusan Koso. 1966. PMID: 5335986 Review. Japanese. No abstract available.
Cited by
-
Neurochemical, structural and neurobehavioral evidence of neuronal protection by whey proteins in diabetic albino mice.Behav Brain Funct. 2015 Feb 13;11:7. doi: 10.1186/s12993-015-0053-0. Behav Brain Funct. 2015. PMID: 25888881 Free PMC article.
-
Effect of duration of diabetes mellitus type 1 on properties of Na, K-ATPase in cerebral cortex.Mol Cell Biochem. 2015 Jul;405(1-2):41-52. doi: 10.1007/s11010-015-2394-2. Epub 2015 Apr 18. Mol Cell Biochem. 2015. PMID: 25893732
-
Insulin deficiency and intranasal insulin alter brain mitochondrial function: a potential factor for dementia in diabetes.FASEB J. 2019 Mar;33(3):4458-4472. doi: 10.1096/fj.201802043R. Epub 2019 Jan 24. FASEB J. 2019. PMID: 30676773 Free PMC article.
-
Asymmetric dimethylarginine inhibits HSP90 activity in pulmonary arterial endothelial cells: role of mitochondrial dysfunction.Am J Physiol Cell Physiol. 2008 Jun;294(6):C1407-18. doi: 10.1152/ajpcell.00384.2007. Epub 2008 Apr 2. Am J Physiol Cell Physiol. 2008. PMID: 18385287 Free PMC article.
-
Diabetes type II: a risk factor for depression-Parkinson-Alzheimer?Neurotox Res. 2011 Feb;19(2):253-65. doi: 10.1007/s12640-010-9203-1. Epub 2010 Jun 15. Neurotox Res. 2011. PMID: 20552313 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources