doi: 10.1016/s1383-5769(99)00020-3.
A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue
Affiliations
- PMID: 11227767
- DOI: 10.1016/s1383-5769(99)00020-3
Item in Clipboard
A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue
Parasitol Int.
2000 Jan.
Abstract
A quantitative colorimetric assay using the oxidation-reduction indicator Alamar Blue was developed to measure cytotoxicity of compounds against the protozoan parasite Leishmania. Absorbance increased linearly with the plating density of promastigotes of L. major MRHO/IR/76 vaccine strain up to at least 2.5 x 10(6) cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue. The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue assay permits a simple, reproducible and reliable method for screening antileishmanial drugs.
Similar articles
-
Susceptibility of clinical isolates of Leishmania aethiopica to miltefosine, paromomycin, amphotericin B and sodium stibogluconate using amastigote-macrophage in vitro model.Exp Parasitol. 2013 May;134(1):68-75. doi: 10.1016/j.exppara.2013.01.022. Epub 2013 Feb 19. Exp Parasitol. 2013. PMID: 23434530
-
Glucantime-resistant Leishmania tropica isolated from Iranian patients with cutaneous leishmaniasis are sensitive to alternative antileishmania drugs.Parasitol Res. 2007 Oct;101(5):1319-22. doi: 10.1007/s00436-007-0638-0. Epub 2007 Jul 5. Parasitol Res. 2007. PMID: 17610079
-
Antileishmanial activity of drug infused mini-agar plates on Leishmania donovani promastigotes.Trop Biomed. 2010 Dec;27(3):657-61. Trop Biomed. 2010. PMID: 21399608
-
Current scenario of drug development for leishmaniasis.Indian J Med Res. 2006 Mar;123(3):399-410. Indian J Med Res. 2006. PMID: 16778319 Review.
-
[Cutaneous leishmaniasis].Hautarzt. 2003 Jun;54(6):506-12. doi: 10.1007/s00105-003-0530-5. Epub 2003 Apr 18. Hautarzt. 2003. PMID: 12759734 Review. German.
Cited by
-
Bromopyrrole alkaloids as lead compounds against protozoan parasites.Mar Drugs. 2010 Jul 14;8(7):2162-74. doi: 10.3390/md8072162. Mar Drugs. 2010. PMID: 20714430 Free PMC article.
-
Anti-Leishmanial and Cytotoxic Activities of a Series of Maleimides: Synthesis, Biological Evaluation and Structure-Activity Relationship.Molecules. 2018 Nov 5;23(11):2878. doi: 10.3390/molecules23112878. Molecules. 2018. PMID: 30400596 Free PMC article.
-
In vitro comparative assessment of different viability assays in Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites.Parasitol Res. 2013 Dec;112(12):4087-95. doi: 10.1007/s00436-013-3599-5. Epub 2013 Sep 12. Parasitol Res. 2013. PMID: 24026387
-
Susceptibility of promastigotes and intracellular amastigotes from distinct Leishmania species to the calpain inhibitor MDL28170.Parasitol Res. 2018 Jul;117(7):2085-2094. doi: 10.1007/s00436-018-5894-7. Epub 2018 May 4. Parasitol Res. 2018. PMID: 29728827
-
Paromomycin: uptake and resistance in Leishmania donovani.Mol Biochem Parasitol. 2009 Apr;164(2):111-7. doi: 10.1016/j.molbiopara.2008.12.007. Epub 2008 Dec 25. Mol Biochem Parasitol. 2009. PMID: 19146886 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
