A novel Rad24 checkpoint protein complex closely related to replication factor C
- PMID: 10660302
- DOI: 10.1016/s0960-9822(99)00263-8
A novel Rad24 checkpoint protein complex closely related to replication factor C
Erratum in
- Curr Biol 2000 Feb 24;10(4):R171
Abstract
Rad24 functions in the DNA damage checkpoint pathway of Saccharomyces cerevisiae. Here, analysis of Rad24 in whole cell extracts demonstrated that its mass was considerably greater than its predicted molecular weight, suggesting that Rad24 is a component of a protein complex. The Rad24 complex was purified to homogeneity. In addition to Rad24, the complex included polypeptides of 40 kDa and 35 kDa. The 40 kDa species was found by mass spectrometry to contain Rfc2 and Rfc3, subunits of replication factor C (RFC), a five subunit protein that is required for the loading of polymerases onto DNA during replication and repair [3]. We hypothesised that other RFC subunits, all of which share sequence homologles with Rad24, might also be components of the Rad24 complex. Reciprocal co-immunoprecipitation studies were performed using extracts prepared from strains containing epitope-tagged RFC proteins. These experiments showed that the small RFC proteins, Rfc2, Rfc3, Rfc4 and Rfc5, interacted with Rad24, whereas the Rfc1 subunit did not. We suggest that this RFC-like Rad24 complex may function as a structure-specific sensor in the DNA damage checkpoint pathway.
Similar articles
-
Rfc5, in cooperation with rad24, controls DNA damage checkpoints throughout the cell cycle in Saccharomyces cerevisiae.Mol Cell Biol. 2000 Aug;20(16):5888-96. doi: 10.1128/MCB.20.16.5888-5896.2000. Mol Cell Biol. 2000. PMID: 10913172 Free PMC article.
-
Functional and physical interaction between Rad24 and Rfc5 in the yeast checkpoint pathways.Mol Cell Biol. 1998 Sep;18(9):5485-91. doi: 10.1128/MCB.18.9.5485. Mol Cell Biol. 1998. PMID: 9710632 Free PMC article.
-
Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway.Mol Cell Biol. 2001 Sep;21(17):5838-45. doi: 10.1128/MCB.21.17.5838-5845.2001. Mol Cell Biol. 2001. PMID: 11486023 Free PMC article.
-
The PCNA-RFC families of DNA clamps and clamp loaders.Prog Nucleic Acid Res Mol Biol. 2004;78:227-60. doi: 10.1016/S0079-6603(04)78006-X. Prog Nucleic Acid Res Mol Biol. 2004. PMID: 15210332 Review.
-
Clamping down on clamps and clamp loaders--the eukaryotic replication factor C.Eur J Biochem. 1998 Jun 1;254(2):209-16. Eur J Biochem. 1998. PMID: 9660172 Review.
Cited by
-
Loss of cell cycle checkpoint control in Drosophila Rfc4 mutants.Mol Cell Biol. 2001 Aug;21(15):5156-68. doi: 10.1128/MCB.21.15.5156-5168.2001. Mol Cell Biol. 2001. PMID: 11438670 Free PMC article.
-
Mec1 and Rad53 inhibit formation of single-stranded DNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutants.Genetics. 2004 Feb;166(2):753-64. doi: 10.1534/genetics.166.2.753. Genetics. 2004. PMID: 15020465 Free PMC article.
-
Genetic and epigenetic features in radiation sensitivity Part I: cell signalling in radiation response.Eur J Nucl Med Mol Imaging. 2005 Feb;32(2):229-46. doi: 10.1007/s00259-004-1730-7. Eur J Nucl Med Mol Imaging. 2005. PMID: 15657757 Review.
-
ATM and ATR: sensing DNA damage.World J Gastroenterol. 2004 Jan 15;10(2):155-60. doi: 10.3748/wjg.v10.i2.155. World J Gastroenterol. 2004. PMID: 14716813 Free PMC article. Review.
-
Fission yeast Rad17 associates with chromatin in response to aberrant genomic structures.Mol Cell Biol. 2001 May;21(10):3289-301. doi: 10.1128/MCB.21.10.3289-3301.2001. Mol Cell Biol. 2001. PMID: 11313455 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
