FIG. 1.

Cloning of the human ERK1c. (A) RT-PCR cloning of ERK1c. The MCF7 lane shows RT-PCR with oligonucleotide primers ERK1-Exon7-S and ERK1-Exon8-AS and total RNA from MCF-7 cells as a template. The left HeLa lane shows the same RT-PCR mixture as in the MCF7 lane, except the RNA was from HeLa cells. The right HeLa lane shows RT-PCR with oligonucleotide primers ERK1-Exon1-S and ERK1c-insert-AS and total RNA from HeLa cells. The positions of the relevant fragments and DNA markers (in base pairs) are indicated at the sides of the gel. (B) cDNA and amino acid sequences of the C-terminal region of ERK1c. The unique ERK1c insert is boxed. (C) Exon organization of ERK1c and ERK1. The length of the open reading frames (ORFs), the number of amino acids (AA), and the predicted molecular mass are indicated. (D) Sequence alignment of human ERK1c, monkey ERK1c, and rat ERK1b proteins. Amino acids that are identical in the different proteins (:) and the percentage identity between the proteins are indicated. Gaps introduced to maximize alignment are indicated by the dashes.