In vivo functional and molecular characterization of the Penicillin-Binding Protein 4 (DacB) of Pseudomonas aeruginosa

Cristian Gustavo Aguilera Rossi; Paulino Gómez-Puertas; Juan Alfonso Ayala Serrano

doi:10.1186/s12866-016-0853-x

Fig. 4

D,D-peptidase activity on purified muropeptides. HPLC chromatograms for digestion assays with purified recombinant protein PBP4HC on natural substrate, M5 (monomer disaccharide pentapeptide), D45 (dimer disaccharide tetrapeptide-pentapeptide), M5N (anhydrous monomer disaccharide pentapeptide) and D45N (anhydrous dimer disaccharide tetrapeptide-pentapeptide). The identified products are labeled: M4, monomer disaccharide tetrapeptide; D44, dimer disaccharide tetrapeptide-tetrapeptide; M4N, anhydrous monomer disaccharide tetrapeptide. A204, absorbance at 204 nm, arbitrary units