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Showing top results for tag:
sam
•
reset
2
votes
5
replies
390
views
ERROR: SAM file generated by BWA is corrupted and cannot be processed with SAMTOOLS
samtools
bwa
NGS
alignment
sam
10 days ago by
Luwell
• 0
2
votes
1
reply
202
views
Filter SAM file for read pairs that align on same strand
samtools
alignment
sam
updated 12 days ago by
Pierre Lindenbaum
165k • written 12 days ago by
kmyers2
▴ 90
1
vote
17
replies
819
views
Unable to get to BAM file from SAM using samtools
Samtools
BAM
SAM
5 days ago by
alejandro.mejiam1
▴ 20
0
votes
0
replies
188
views
Converting gaf files to sam/bam?
bam
gaf
sam
26 days ago by
guntul
▴ 40
2
votes
7
replies
869
views
6 follow
Parsing error in sam file when converting to bam
samtools
bwa
fastq
bam
sam
updated 4 weeks ago by
GenoMax
149k • written 5 weeks ago by
emily.coger
• 0
2
votes
5
replies
558
views
MISO Sorted BAM file header mismatch
bam
miso
sam
minimap2
updated 12 weeks ago by
Istvan Albert
102k • written 3 months ago by
bimeva5703
• 0
1
vote
1
reply
410
views
Can a read be aligned more than once?
alignment
gaf
read
sam
updated 3 months ago by
GenoMax
149k • written 3 months ago by
guntul
▴ 40
4
votes
2
replies
393
views
Converting bam to sam using htslib C++ api
bam
htslib
sam
updated 4 months ago by
Pierre Lindenbaum
165k • written 4 months ago by
Uveyik
▴ 70
4
votes
1
reply
335
views
Extract bam file to sam inside code?
bam
sam
updated 4 months ago by
Pierre Lindenbaum
165k • written 4 months ago by
Uveyik
▴ 70
3
votes
2
replies
467
views
Difference between gam and sam/bam files?
bam
pangenome
gam
sam
4 months ago by
Uveyik
▴ 70
1
vote
1
reply
402
views
When to use Sam Sort on a Bam file?
Sam
Bam
Sort
File
Collate
updated 7 months ago by
Pierre Lindenbaum
165k • written 7 months ago by
Arthur
▴ 10
0
votes
1
reply
428
views
Difference between samtools stats insert size and TLEN in SAM/BAM file.
samtools
insert-size
SAM
updated 7 months ago by
Ram
44k • written 7 months ago by
krzysm20009042
• 0
0
votes
3
replies
658
views
Sam file Header problem
Sam
Header
problem
file
updated 9 months ago by
Pierre Lindenbaum
165k • written 9 months ago by
saifulislam99121
• 0
1
vote
3
replies
1.1k
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
9 months ago by
kalavattam
▴ 310
0
votes
3
replies
671
views
Difference between samtools "-f 9" and "-f 11"
samtools
sam
bam
updated 10 months ago by
Istvan Albert
102k • written 10 months ago by
Dylan
• 0
4
votes
2
replies
2.8k
views
How to convert SAM/BAM file to GTF/GFF file?
GTF
SAM
BAM
GFF
minimap2
updated 10 months ago by
Arslan Tariq
▴ 130 • written 22 months ago by
BioinfoBee
• 0
1
vote
1
reply
487
views
How do I get the 3bp sequence on either side of a co-ordinate
SAM
genomics
sequencing
updated 10 months ago by
Ram
44k • written 10 months ago by
nrav58
• 0
0
votes
1
reply
366
views
BRIG coverage.graph issues
BRIG
sam
updated 11 months ago by
Pierre Lindenbaum
165k • written 11 months ago by
dlera.lozano
▴ 10
2
votes
5
replies
1.2k
views
samtools mpileup taking too long
Bam
samtools
sam
alignment
updated 10 months ago by
Ram
44k • written 11 months ago by
K
• 0
7
votes
5
replies
3.8k
views
What is chr EBV in human sam file?
SAM
Bowtie2
written 6.6 years ago by
salamandra
▴ 550
2
votes
1
reply
921
views
How to get unaligned reads and aligned reads into separate files from SAM/BAM?
bam
sam
reads
fastq
updated 14 months ago by
rpolicastro
13k • written 14 months ago by
O.rka
▴ 740
1
vote
7
replies
1.4k
views
Samtools filtering based on PNEXT
CRAM
filtering
samtools
SAM
BAM
updated 15 months ago by
Istvan Albert
102k • written 15 months ago by
predeus
★ 2.1k
1
vote
1
reply
928
views
Filtering for primary and secondary reads using sam flags (0 properly paired reads in alignment step)
freebayes
samtools
samflags
sam
paired-end
updated 16 months ago by
aw7
▴ 350 • written 16 months ago by
Marcel Glueck
▴ 10
1
vote
4
replies
1.9k
views
bowtie mismatch 0 alignment output
sequencing
bam
sam
bowtie
rna-seq
updated 16 months ago by
feng10749
• 0 • written 3.7 years ago by
kb_93
▴ 10
0
votes
1
reply
485
views
Does GATK SetNmMdAndUqTags reduces the size of a CRAM?
CRAM
WGS
sam
GATK
SetNmMdAndUqTags
updated 16 months ago by
Pierre Lindenbaum
165k • written 16 months ago by
Sd
• 0
4
votes
6
replies
2.9k
views
6 follow
How to discard alignments from SAM/BAM file that have mismatches
BAM
SAM
minimap
nanopore
updated 16 months ago by
Brian Bushnell
20k • written 20 months ago by
Mo
▴ 50
5
votes
12
replies
2.1k
views
sam format in the emboss water program
emboss
alignment
sam
water
updated 16 months ago by
John Marshall
3.1k • written 16 months ago by
Malcolm.Cook
★ 1.5k
2
votes
3
replies
939
views
Finding exactly where reads map from SAM
alignment
read
Bowtie
SAM
BWA
updated 16 months ago by
Istvan Albert
102k • written 16 months ago by
Sisyphus
• 0
1
vote
1
reply
592
views
Bam sort merge error?
bam
linux
sam
updated 16 months ago by
Pierre Lindenbaum
165k • written 16 months ago by
Athena
• 0
7
votes
4
replies
1.0k
views
BAM file read stats (A/T/C/G/ins/del) for each base position
SAM
BAM
Nanopore
JBrowse
updated 17 months ago by
Dave Carlson
★ 2.1k • written 17 months ago by
herrenc
▴ 20
5
votes
8
replies
5.4k
views
Distinguish between forward strand, plus strand, sense strand
pysam
strand
star
sam
updated 17 months ago by
Buwei
• 0 • written 2.6 years ago by
tien
▴ 40
0
votes
1
reply
552
views
How to get MD tag sequence of a read from a sam file given a bed file?
samtools
bed
sam
cigar
updated 18 months ago by
aw7
▴ 350 • written 18 months ago by
n.a.tekkey
• 0
0
votes
2
replies
865
views
How to build a tree from SAM files?
SAM
phylogeny
alignment
18 months ago by
Alina
• 0
2
votes
5
replies
1.1k
views
Help with htseq -count read counts
sam
gtf
RNA-seq
htseq
updated 19 months ago by
rfran010
★ 1.3k • written 19 months ago by
Juan Pablo
• 0
2
votes
2
replies
811
views
Are SAM attributes necessary if I only keep the BAM files using STAR?
BAM
SAM
RNA-seq
STAR
updated 19 months ago by
GenoMax
149k • written 19 months ago by
ev97
▴ 40
4
votes
5
replies
1.1k
views
Samtools sort error: [pretty_header] invalid header
sam
RNA-seq
bam
samtools
updated 20 months ago by
Ram
44k • written 20 months ago by
Ondina
▴ 100
2
votes
2
replies
770
views
Identify similarly mapped reads in BAM/SAM?
bam
igv
sam
python
r
20 months ago by
Eliveri
▴ 350
9
votes
5
replies
6.4k
views
Decoding MM and ML tags in .SAM file
samtools
Methylation
SAM
updated 13 months ago by
June
▴ 20 • written 21 months ago by
Ethan
▴ 20
1
vote
6
replies
3.7k
views
How to extract alignments with 0 mismatch from sam or bam file by using samtools?
bam
samtools
sam
updated 21 months ago by
Hien
• 0 • written 3.7 years ago by
khanhlpbao
• 0
0
votes
0
replies
1.0k
views
Invalid Contig error when using bamfile.fetch
sam
Pysam
bam
python
21 months ago by
bxxxmxxxx
▴ 10
3
votes
3
replies
2.5k
views
base modifications tags in SAM file
methylation
SAM
tag
nanopore
bam
updated 8 months ago by
colindaven
7.1k • written 21 months ago by
antoine.fauchois92
▴ 20
1
vote
3
replies
1.1k
views
".sam" file - should it contain multiple samples?
SAM
updated 22 months ago by
GenoMax
149k • written 22 months ago by
Roland
▴ 20
3
votes
6
replies
2.0k
views
bam index chromosome size limit
bam
sam
mapping
rna-seq
updated 19 months ago by
Chen-Jui
• 0 • written 22 months ago by
николай
• 0
3
votes
4
replies
1.2k
views
How to filter bam file for only reads that span certain regions of the genome?
reference-genome
bam
sam
bedtools
updated 22 months ago by
Ram
44k • written 22 months ago by
kaufman.eli.j
• 0
0
votes
2
replies
924
views
Which ATAC-seq SAM/BAM fields are required for bigwig generation by deeptools bamCoverage?
bamCoverage
BAM
SAM
deeptools
updated 20 months ago by
Ram
44k • written 22 months ago by
Deep
• 0
3
votes
3
replies
1.5k
views
how can i filter a sam file from a paf file
paf
sam
filter
updated 22 months ago by
ATpoint
87k • written 23 months ago by
Mark
▴ 30
1
vote
2
replies
1.7k
views
Best way to visualize .paf or .sam alignment to a fasta file
visualization
sam
fasta
paf
alignment
updated 23 months ago by
colindaven
7.1k • written 23 months ago by
Mark
▴ 30
0
votes
6
replies
1.2k
views
Mapping paired end reads with ngm and samtools, using prefixes and suffixes for creating vcf eventually
ngm
sam
samtools
updated 23 months ago by
Ram
44k • written 23 months ago by
Human
• 0
0
votes
0
replies
518
views
Add HI:i:<n> tag to a BAM file
SAM
hisat2
STAR
tag
BAM
23 months ago by
predeus
★ 2.1k
2
votes
2
replies
4.3k
views
BWA alignment score calculation
score
SAM
BWA
alignment
updated 23 months ago by
kmzhou4
• 0 • written 3.7 years ago by
Donovan
▴ 20
50 results • Page
1 of 1
Recent Votes
Comment: Differential Expression and absolute expression
Is there a tool or method for phasing a VCF based on an already phased BAM?
A: FindConservedMarkers vs FindMarkers vs FindAllMarkers Seurat
Answer: Differential Expression and absolute expression
Comment: circos ignoring the ticks and plots
Answer: Split genotypes in VCF file
C: Plotting numbers of features in EBImage in R
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Comment: Unusual File Count in 10x scMultiome Data for Cell Ranger ARC Processing
by
Wu-Sheng Zhang
• 0
Hi @genomax , thank you very much for your help! I go through that page but still a little confused. 10x scMultiomics data would store scAT…
Comment: multiBamSummary
by
JC
13k
check https://broadinstitute.github.io/picard/explain-flags.html to choose a proper flag
Answer: multiBamSummary
by
JC
13k
Yes, `--samFlagExclude 16` will exclude reads in the reverse strand, you need `--samFlagInclude 16` (primary hits, reverse strand) or some …
Answer: Mapping ~100-300bp Sequences to Metagenome-Assembled Genomes
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Mensur Dlakic
★ 29k
While I use Bowtie2, there is not a strong argument for it versus other genome aligners. I guess it is because I found a pipeline back in t…
Comment: How to convert CIGAR string to VCF?
by
cmdcolin
★ 4.1k
i think this is a good exercise (I wrote a quick code that I have used for cigar parsing in javascript https://cmdcolin.github.io/posts/202…
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Jeremy Leipzig
23k
seqr now fully supports Hail but apparently there are some issues that will force yet another switch https://github.com/broadinstitute/seqr…
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165k
pseudo code: refpos1 = <something-clipped-start> readpos0= 0; for(CigarElement ce: cigar) { char co = ce.getOpera…
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3.2k
vt decompose did the trick for me, followed by the filtering for 10th and 11th column. Thanks!
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149k
Looks good to me.
Comment: CLI Downloading from Human Microbiome Project Given Login + contact failure
by
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3.3k
Try the [HMP client][1] to download files from their [new portal][2]. [1]: https://github.com/IGS/portal_client [2]: https://portal.h…
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i used the following command. Kindly let me know if i missed something or if it is now correct ``` bbsplit.sh ref=human_GRCh38.p14_genomic…
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Thank you! I finally used some code from my supervisor for the Lorenz curves, it's relatively easy, and DescTools::Gini to compute the gini…
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dthorbur
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I'm not all that familiar with your field of research, but it would seem counterproductive to try and assemble reads of a microbial communi…
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149k
It is worth trying. You will know reads that map to the fungal reference (even though fragmented) definitely belong there. You could also…
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unfortunatly there is no chromosoem level reference genome available. But there are some genome assemblies available at scaffold or contig …
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