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50 results • Page
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Showing top results for tag:
paired-end
•
reset
2
votes
3
replies
379
views
Paired-end sequencing with inconsistent quality/number of reads
paired-end
NGS
STAR
flowcell
alignment
4 days ago by
anissa
• 0
0
votes
3
replies
369
views
Cross-strand correlation for paired-end ChIP-seq data
paired-end
ChIP-seq
RelCC
single-end
updated 4 weeks ago by
LChart
4.3k • written 4 weeks ago by
alison.ludzki
• 0
0
votes
1
reply
315
views
Integration of paired and single-end SRA
Paired-end
Single-end
VCF
Resequencing
updated 6 weeks ago by
Brian Bushnell
20k • written 6 weeks ago by
reza
▴ 300
2
votes
4
replies
356
views
One SRR (one fastq) file for pair-end layout
SRR
paired-end
SRA
12 weeks ago by
Vojtěch
▴ 10
4
votes
6
replies
577
views
How to check if all read names are paired properly in paired end fastq.gz files?
samtools
illumina
paired-end
updated 3 months ago by
LChart
4.3k • written 3 months ago by
DNAngel
▴ 250
1
vote
2
replies
313
views
How to figure out adapter sequence to use for trimming old miRNAseq data
paired-end
fastq
rna-seq
mirna-seq
trimming
3 months ago by
hsothers
• 0
1
vote
3
replies
799
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
5 months ago by
kalavattam
▴ 270
6
votes
4
replies
13k
views
Trimmomatic: What is the difference between paired and unpaired output files in paired-end mode
trimmomatic
paired-end
updated 5 months ago by
RT
• 0 • written 6.9 years ago by
dllopezr
▴ 130
1
vote
4
replies
987
views
Understanding STAR output (Aligned.out.sam file)
STAR
paired-end
read
5 months ago by
heelpPlease
• 0
3
votes
7
replies
708
views
FastQC Quality per tile and per sequence behaving strange after using Cutadapt
fastqc
cutadapt
illumina
tile
paired-end
6 months ago by
Sergio A.S.
▴ 10
4
votes
4
replies
599
views
Is it feasible to merge Illumina RNA-Seq data with varying read lengths?
illumina
RNA-seq
paired-end
updated 7 months ago by
i.sudbery
20k • written 7 months ago by
mathavanbioinfo
▴ 80
2
votes
5
replies
931
views
Weird Fragment Length distribution multiQC
Salmon
RNA-seq
paired-end
MultiQC
updated 7 months ago by
GenoMax
146k • written 8 months ago by
_deb
• 0
0
votes
1
reply
349
views
Which FLASH parameters to use?
paired-end
flash
single-end
merged
updated 8 months ago by
GenoMax
146k • written 8 months ago by
Elisa
• 0
0
votes
2
replies
706
views
invalid deflate data (invalid code lengths set)
Paired-End
Trimming
Fastq
TrimGalore
updated 9 months ago by
Ram
44k • written 9 months ago by
leonmcswain
▴ 10
9
votes
7
replies
1.6k
views
Trying to wrap my head around Illumina paired end sequencing
paired-end
illumina
sequencing
updated 9 months ago by
Istvan Albert
101k • written 9 months ago by
ScottDansk
▴ 10
3
votes
7
replies
1.1k
views
"C++ compiler cannot create executables", while installing ABySS 2.0 assembler
assembly
illumina
abyss
btllib
paired-end
updated 9 months ago by
Raony Guimarães
★ 1.4k • written 10 months ago by
Vijith
▴ 60
2
votes
1
reply
444
views
Shorten Illumina reads
RepeatExplorer2
Illumina
paired-end
updated 11 months ago by
Brian Bushnell
20k • written 11 months ago by
kirillkirilenko
▴ 40
0
votes
2
replies
506
views
Doubt about RNASeq Paired-end process
RNASEQ
NGS
Paired-end
11 months ago by
Vicent
• 0
0
votes
0
replies
342
views
Query about ALLHiC tutorial data format
allhic
paired-end
12 months ago by
mbeavitt
• 0
1
vote
1
reply
730
views
Filtering for primary and secondary reads using sam flags (0 properly paired reads in alignment step)
freebayes
samtools
samflags
sam
paired-end
updated 12 months ago by
aw7
▴ 340 • written 12 months ago by
Marcel Glueck
▴ 10
1
vote
1
reply
711
views
bedGraph for Coverage of Insert from Paired-End Data?
genome
awk
coverage
bed
paired-end
updated 13 months ago by
shelkmike
★ 1.4k • written 13 months ago by
vanbelj
▴ 40
6
votes
8
replies
1.3k
views
Quantify the number of sequences in common paired-end sequencing data
fastq
paired-end
updated 13 months ago by
Ram
44k • written 13 months ago by
sil_bioinfo
▴ 50
2
votes
7
replies
1.7k
views
VG mapping paired-end reads: error [xg]: multiple hits for XXX
variation-graph
reads
pangenome
vg
paired-end
14 months ago by
nkls063408
• 0
2
votes
7
replies
1.1k
views
Automatically detecting paired end reads.
pipeline
automation
paired-end
single-end
fastq
updated 16 months ago by
Ram
44k • written 16 months ago by
sam.himes92
• 0
4
votes
5
replies
14k
views
Take A Subset Of A Fastq Paired-End Sample
paired-end
fastq
rna-seq
illumina
updated 17 months ago by
Ram
44k • written 11.6 years ago by
dfernan
▴ 770
2
votes
4
replies
1.2k
views
Different total number of sequences between the two PE fastq files
NGS
RNA-Seq
Paired-End
updated 17 months ago by
GenoMax
146k • written 17 months ago by
Sz
• 0
4
votes
7
replies
1.3k
views
Combining data from single- and paired-end sequencing done on same samples
bulk
Illumina
RNA-seq
System
Ovation
single-end
NextSeq
550
paired-end
Universal
18 months ago by
tise.suz
▴ 10
0
votes
1
reply
724
views
Discordinant aligment
Paired-end
HISAT2
updated 19 months ago by
Istvan Albert
101k • written 19 months ago by
ali
• 0
0
votes
1
reply
416
views
poly A in paired end reads
polyA
paired-end
updated 20 months ago by
Ram
44k • written 20 months ago by
שיר
• 0
1
vote
2
replies
985
views
BBMap/sh/repair.sh not finding pairs
BBMap
fastq
repair
paired-end
repair.sh
updated 20 months ago by
Matthias Zepper
4.9k • written 20 months ago by
Eliott
• 0
5
votes
3
replies
1.1k
views
Paired - end sequencing
NGS
Paired-end
Illumina
updated 22 months ago by
Prash
▴ 280 • written 22 months ago by
adarsh_munna
▴ 50
0
votes
5
replies
1.2k
views
Filter only overlapping portion of read pairs?
bisulfite
paired-end
updated 22 months ago by
Jack Tierney
▴ 410 • written 22 months ago by
Tom Ellis
• 0
4
votes
0
replies
752
views
Convert ONE pair-end BAM file to ONE single-end BAM file with merged mates (i.e. merging the paired reads coverage)
MACS2
BAM
paired-end
single-end
updated 15 months ago by
Ram
44k • written 24 months ago by
Chilly
★ 1.3k
1
vote
2
replies
762
views
Low merging rate of 16S PE reads, possibly overrepresented seqs?
FastQC
Illumina
16S
overrepresented
paired-end
2.0 years ago by
A
• 0
2
votes
2
replies
1.3k
views
Concatenate multiple paired-end reads or interleaved which is best approach to align with BWA-mem2
interleaved
paired-end
bwa-mem2
2.1 years ago by
ben@f
▴ 20
17
votes
6
replies
2.0k
views
Mutect2 produces 0 results for somatic mutation calling of paired-end reads (BAM)
Mutect2
RNA-seq
mutation
paired-end
variant
2.1 years ago by
Chilly
★ 1.3k
14
votes
8
replies
19k
views
TrimGalore! on multiple paired fastq files
rna-seq
trimgalore!
paired-end
script
updated 23 months ago by
Ram
44k • written 8.1 years ago by
emblake
▴ 90
0
votes
0
replies
355
views
Unampped paired-end reads in STAR
Paired-End
RNA-Seq
STAR
Alignment
PE
2.1 years ago by
ilovesuperheroes1993
▴ 40
4
votes
3
replies
2.3k
views
bbduk paired-end RNA-seq trimming command
rna-seq
paired-end
bbduk
trimming
adapter
updated 2.2 years ago by
GenoMax
146k • written 2.2 years ago by
predeus
★ 2.0k
2
votes
3
replies
869
views
mapping paired end data
paired-end
bam
bowtie
mapping
updated 19 months ago by
Ram
44k • written 2.2 years ago by
upasana
▴ 20
1
vote
1
reply
545
views
Getting .bed file
fastq
bed
paired-end
updated 2.2 years ago by
Asaf
10k • written 2.2 years ago by
upasana
▴ 20
2
votes
13
replies
3.7k
views
[RNAseq, featureCounts] Can paired-end data be processed as single end?
paired-end
RNAseq
featureCounts
2.2 years ago by
Texx
• 0
1
vote
2
replies
1.3k
views
Matching R1 and R2 in paired end reads from Illumina
illumina
reads
paired-end
2.3 years ago by
Priyanka
▴ 10
13
votes
8
replies
1.9k
views
6 follow
paired end mapping with one end being unique and the other end multiple
paired-end
genome
ngs
updated 2.1 years ago by
d-cameron
★ 2.9k • written 2.5 years ago by
schu
• 0
1
vote
1
reply
881
views
How to do Fastq quality control on paired-end samples?
QC
paired-end
fastqc
rna-seq
updated 2.5 years ago by
ATpoint
85k • written 2.5 years ago by
gbmygbm
▴ 10
0
votes
1
reply
579
views
Paired-end dataset from SRA database
paired-end
sra
updated 2.5 years ago by
ATpoint
85k • written 2.5 years ago by
melissachua90
▴ 70
0
votes
6
replies
3.4k
views
Replacing Illumina index in header of reads
next-gen-sequencing
paired-end
updated 2.3 years ago by
Ram
44k • written 6.9 years ago by
john.soghigian
• 0
0
votes
0
replies
375
views
bowtie2 paired-end behavior where one mate aligns uniquely and one mate aligns multiple times?
paired
paired-end
bowtie2
multiple
2.7 years ago by
Ryan
• 0
1
vote
3
replies
2.0k
views
how to align paired and unpaired fastq files of a sample using STAR?
Paired-end
STAR
Alignment
updated 2.8 years ago by
swbarnes2
14k • written 2.8 years ago by
milad eidi
▴ 20
1
vote
1
reply
568
views
Paired-end sequnces of 16S rRNA are not merged.
Flash
paired-end
rRNA
sequnces
16S
updated 2.9 years ago by
colindaven
6.8k • written 2.9 years ago by
shabegmatov
• 0
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