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Showing top results for tag:
16s
•
reset
0
votes
0
replies
198
views
spike-in-methods in 16s microbial analysis
16s
spike-in
microbiome
10 days ago by
mrashad
▴ 80
3
votes
5
replies
443
views
Reporting novel species from environmental samples
bacteria
16S
genomics
updated 21 days ago by
Ram
44k • written 24 days ago by
Dunois
★ 2.6k
2
votes
5
replies
419
views
Trimmomatic in windows
windows
16S
trimmomatic
updated 9 weeks ago by
GenoMax
145k • written 9 weeks ago by
Clíona
• 0
1
vote
2
replies
506
views
Trimming adapters and primer on ONT nanopore sequencing
trimming
16S
nanopore
3 months ago by
l.gallucci
▴ 20
2
votes
8
replies
551
views
Are there instutions that allow to use their hpc without charge?
16s
hpc
Nanopore
updated 3 months ago by
a.alnawfal.1992
▴ 350 • written 3 months ago by
kerianaleerivera
• 0
0
votes
1
reply
169
views
Forum:
Are bacterial 16s paralogs real, and do they bias microbiome analyses conducted with QIIME2?
QIIME
paralog
microbiome
bias
16s
4 months ago by
evozoa
• 0
0
votes
0
replies
256
views
16S analysis for specie of bacteria identification
Species-identification
16S
Kraken2
updated 4 months ago by
Ram
44k • written 4 months ago by
Christopher
▴ 10
5
votes
3
replies
495
views
FastQC interpretation - 16S sequencing
interpretation
amplicon
Fastqc
16S
updated 4 months ago by
GenoMax
145k • written 4 months ago by
Christopher
▴ 10
0
votes
1
reply
534
views
16S rRNA microbiome differential abundance analysis with DESeq2 finds same microbial Genus in both groups.
microbiome
16S
DESeq2
updated 4 months ago by
andres.firrincieli
3.7k • written 4 months ago by
Antonio
• 0
3
votes
8
replies
1.0k
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 4 months ago by
Chris Dean
▴ 410 • written 5 months ago by
sovrappensiero
▴ 100
0
votes
0
replies
313
views
Tool:
MicrobiomePhylo: A Comprehensive Platform for Advanced Microbiome Amplicon Sequencing Data Analysis and Visualization
microbiome
analysis
data
16S
6 months ago by
camillatafuro
• 0
1
vote
4
replies
471
views
BLAST parameters for sequences with high similarity
BLAST
Sanger
16S
v4
miseq
updated 6 months ago by
GenoMax
145k • written 6 months ago by
behzad.karkaria
• 0
0
votes
0
replies
376
views
Tool:
Microbiome Analysis: Comprehensive 16S Meta-barcoding Analysis in R with Phyloseq - On Sale Now!
R
microbiome
16S
phyloseq
analysis
7 months ago by
camillatafuro
• 0
2
votes
5
replies
902
views
Trouble Understanding DESeq2 Design Formula ("Model Matrix is not Full Rank")
deseq2
biomarkers
16S
7 months ago by
Rachel
• 0
1
vote
2
replies
653
views
16S amplicon sequencing input data (spreadsheet): barcode vs. linker primer seqs?
barcode
amplicon
16s
9 months ago by
sovrappensiero
▴ 100
2
votes
3
replies
650
views
Less number of total Species than Genus in 16S data
microbiome
Species
Genus
ASV
16S
updated 9 months ago by
Philipp Bayer
8.7k • written 9 months ago by
arshad1292
▴ 110
0
votes
0
replies
289
views
Comparing 16S rRNA (culture) sequences with metagenome
metagenome
shotgun
rRNA_culture
16S
updated 10 months ago by
Ram
44k • written 10 months ago by
aswin
• 0
3
votes
5
replies
1.8k
views
kraken2 bacteria database 250GB+
metagenomics
16s
kraken2
kraken
updated 10 months ago by
GenoMax
145k • written 10 months ago by
10mz1
▴ 10
0
votes
4
replies
721
views
very few sequences classified with Kraken2
metagenomics
16s
kraken2
kraken
updated 10 months ago by
dthorbur
★ 2.4k • written 10 months ago by
10mz1
▴ 10
1
vote
2
replies
737
views
mv Question during kraken2-build (building database)
16s
metagenomics
kraken
kraken2
10 months ago by
10mz1
▴ 10
0
votes
3
replies
1.0k
views
Phyloseq and ASV names between datasets
dada2
phyloseq
16S
asv
updated 11 months ago by
antonioggsousa
3.2k • written 11 months ago by
pablo
▴ 310
0
votes
7
replies
1.3k
views
What is wrong with my NCBI esearch command for 16S rRNA?
16S
NCBI
Entrez
assembly
updated 12 months ago by
GenoMax
145k • written 12 months ago by
Morgan S.
▴ 90
1
vote
2
replies
727
views
functional analysis
16S
metabarcoding
functionalanalysis
12 months ago by
safeassli
▴ 10
3
votes
3
replies
991
views
When counting ASVs in DADA2, does a hit/count only occur at 100% alignment percent identity?
metagenomics
qiime2
amplicon
16s
dada2
updated 13 months ago by
andres.firrincieli
3.7k • written 13 months ago by
O.rka
▴ 740
0
votes
2
replies
797
views
should remove unassigned genera in 16S rRNA sequencing analysis
16S
rRNA
metagenomics
sequencing
14 months ago by
zhangdengwei
▴ 210
0
votes
2
replies
994
views
Question about 16S rRNA reads we got from a sequencing company
16S
adapters
metabarcoding
primers
amplicon
15 months ago by
KQUB
• 0
1
vote
0
replies
441
views
Alternative approach to rarefying in 16S rRNA analysis
16S
Rarefying
rRNA
16 months ago by
zhangdengwei
▴ 210
1
vote
1
reply
774
views
Assigned taxonomy after dada2 only "NAs" or "Mitochondria"
16S
dada2
DECIPHER
ITS
taxonomy
16 months ago by
mattze731
▴ 20
0
votes
2
replies
963
views
too many zeros in 16S rRNA amplicon sequencing data
16S
rRNA
16 months ago by
zhangdengwei
▴ 210
0
votes
1
reply
1.1k
views
Tool:
web app for user-friendly analysis of 16S rRNA gene meta-barcoding NGS data - bacterial microbiome
qiime2
16s
microbiome
ngs
updated 16 months ago by
Ram
44k • written 17 months ago by
camillatafuro
• 0
0
votes
2
replies
676
views
Is there a SILVA database version with only prokaryotes?
taxonomy
SILVA
16S
17 months ago by
SushiRoll
▴ 130
0
votes
0
replies
402
views
Inferring relative gene abundance from short reads
metagenomics
read
16S
short
mapping
17 months ago by
SushiRoll
▴ 130
5
votes
3
replies
928
views
How do i search a FASTA database by sequence in seqkit?
seqkit
rRNA
genomics
16s
gene
updated 17 months ago by
GenoMax
145k • written 17 months ago by
Mahmoud Reda
▴ 10
2
votes
2
replies
743
views
Annotation of 16S rDNA identified bacteria
rDNA
16s
updated 19 months ago by
Mensur Dlakic
★ 28k • written 19 months ago by
Mahnoor
• 0
0
votes
2
replies
725
views
Miseq mothur error with SOP in galaxy - Help please!
mothur
miseq
16s
Galaxy
sop
updated 20 months ago by
colindaven
6.7k • written 20 months ago by
Melissa
• 0
0
votes
2
replies
799
views
Number of samples for lefse analysis
16s
Lefse
metagenome.
20 months ago by
Sowmya Pulapet
▴ 70
0
votes
0
replies
352
views
Trying to compare 16S taxonomies for different samples post RDP classifier with R
metagenomics
16S
taxonomy-classifier
RDP
R
updated 20 months ago by
Ram
44k • written 20 months ago by
Elvira
• 0
0
votes
0
replies
535
views
News:
Metabarcoding in Microbial Ecology
Metabarcoding
Qiime2
16S
MicrobialEcology
R
20 months ago by
carlopecoraro2
★ 2.6k
0
votes
0
replies
508
views
Metagenome analysis on EPI2ME ONT
metagenomics
ONT
16S
longreads
21 months ago by
kirillkirilenko
▴ 40
1
vote
2
replies
689
views
16s sequence Identification
Identification
16s
sequence
updated 23 months ago by
5heikki
11k • written 23 months ago by
saifulislam99121
• 0
0
votes
2
replies
765
views
Pairwise percent identity matrix generation?
16S
sequencing
amplicon
percent
identity
updated 23 months ago by
GenoMax
145k • written 23 months ago by
pearl2070
▴ 10
0
votes
3
replies
804
views
What algorithm to use to align sequences of gene to reference gene and analyze variants?
RNASeq
16S
CRISPR
Sequencing
Fastq
updated 23 months ago by
GenoMax
145k • written 23 months ago by
Saran
▴ 50
1
vote
2
replies
748
views
Low merging rate of 16S PE reads, possibly overrepresented seqs?
FastQC
Illumina
16S
overrepresented
paired-end
23 months ago by
A
• 0
1
vote
0
replies
480
views
Identification of V1 to V9 regions 16S
V9
V1
16S
identification
updated 24 months ago by
Matthias Zepper
4.9k • written 24 months ago by
A_heath
▴ 160
12
votes
8
replies
1.6k
views
Align 16S sequence to a reference
16S
sequence
alignment
updated 2.0 years ago by
Matthias Zepper
4.9k • written 2.1 years ago by
A_heath
▴ 160
5
votes
1
reply
1.6k
views
To create a pre-trained classifier provided by QIIME2 using RESCRIPt
RESCRIPt
rRNA
16S
Qiime2
SILVA
updated 2.1 years ago by
andres.firrincieli
3.7k • written 2.1 years ago by
yeshirata
▴ 20
1
vote
1
reply
710
views
qiime2 issues about taxonomy file
Qiime2
SILVA
rRNA
16S
updated 2.1 years ago by
andres.firrincieli
3.7k • written 2.1 years ago by
yeshirata
▴ 20
0
votes
3
replies
2.0k
views
Are there any tools that automatically detect where to truncate forward and reverse reads for 16S (DADA2)?
16s
dada2
illumina
metagenomics
qiime2
2.1 years ago by
O.rka
▴ 740
0
votes
1
reply
610
views
Identification of conserved and varaible regions on 16s rRNA sequences
16s
illumina
sequencing
NGS
updated 2.3 years ago by
Chris Dean
▴ 410 • written 2.3 years ago by
Robert
• 0
2
votes
9
replies
2.1k
views
Using kraken2 to fish out only 16S reads
microbiome
metagenomics
16S
Deep-sequencing
updated 2.3 years ago by
Mensur Dlakic
★ 28k • written 2.3 years ago by
Rezenman
• 0
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