We are starting making our own library for mRNA sequencing, and we are totally clueless about what adapter sequence to use! I have gone through the Illumina adapter sequences on their websites, but there are larger sequences then what my kit provider (Vazyme) is offering. Any one can tell me about how to start with adapter sequences and what adapter sequence to add, we will be using Novaseq 6000 machine and PE150 mode Thanks
Before preparing your libraries, there are some QC steps for sequencing cores to ensure that your samples are suitable for sequencing. For example, one of the criteria is the RIN score. I recommend reaching out to a sequencing core, vendors, or Illumina to confirm that the quality of your samples meets their QC criteria.