how to design primer with primer3 and what parameters are necessary to feed to primer3

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2.9 years ago

eli_bayat ▴ 90

Hello, I need to design left and right primers and this is my first time doing it. I have very basic questions and appreciate any help.

I noticed primer3 is a nice tool to design primers, but it comes with different parameters to tune. So my questions are:

What information I need for designing primers, in other words what are the "must feed in" parameters to the primer3.
I think in order to set up some of the parameters such as annealing temperature, concentration or amplicon size, I need to ask lab scientist who will be using these primers. I would appreciate any help on selection of the parameters that I need to ask lab scientists to provide me before designing primers.

Thanks

primer_design design primers primer3 • 994 views

ADD COMMENT • link 2.9 years ago by eli_bayat ▴ 90

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I think in order to set up some of the parameters such as annealing temperature, concentration or amplicon size, I need to ask lab scientist

well, ... I think you should start with this.

ADD REPLY • link 2.9 years ago by Pierre Lindenbaum 164k

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okay, thank you, I wanted to make sure this is the first step.

ADD REPLY • link 2.9 years ago by eli_bayat ▴ 90

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