This repository is for the analyses of the following studies:

Choi, M.-K., Cook, A., Mungikar, K., Eachus, H., Tochwin, A., Linke, M., Gerber, S., Ryu, S., Exposure to elevated glucocorticoid during development primes altered transcriptional responses to acute stress in adulthood, ISCIENCE (2024), doi: https://doi.org/10.1016/ j.isci.2024.110160.

Eachus, H., Choi, MK., Tochwin, A. et al. Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish. Commun Biol 7, 416 (2024). https://doi.org/10.1038/s42003-024-06060-5.

Backgrounds of sequencing. paired-end TruSeq Stranded mRNA libraries (Illumina, CA, USA) were constructed and over 20M of 50 bp reads/sample were sequenced. For this study, a total of 60 brain samples were sequenced, consisting of 5 biological replicates at four different time points (6, 13, 120 dpf, and acute-stressed at 120dpf) in each of three groups (wildtype (WT), bPAC+, and bPAC-). Raw sequenced reads(.fastq) have been deposited in ENA (PRJEB53713).