CRISPR Barista is a simple UI to drive your command line CRISPResso2 program. The program uses Python3.7+, Flask and Boostrap.

CRISPResso2 must be installed using bioconda method (running CRISPResso2 -h must not fail).

Python3.7+ must be installed in a Unix-like operating system (MacOS or Linux).

Handle only those parameters: --fastq_r1, --fastq_r2, --amplicon_seq and --guide_seq

In case you have a single fastq file containing 2 reads, you can check split, the program will split the files for you.

Initialize a local environment and install dependencies. Python3 is required.

Launches the application. Once you see * Running on http://127.0.0.1:5000 (Press CTRL+C to quit) open http://127.0.0.1:5000 with your favorite browser.

Warning: do not deploy in production, use only localy

Launches the application in debug mode.

Main interface

Form filled using a double reads fastq file

CRISPResso2 results