A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel biological applications. However, before stepping into the limelight, next-generation sequencing had to overcome the inertia of a field that relied on Sanger-sequencing for 30 years.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Highly Aligned Ternary Nanofiber Matrices Loaded with MXene Expedite Regeneration of Volumetric Muscle Loss
Nano-Micro Letters Open Access 04 January 2024
-
Co-occurrence of Spondyloepiphyseal Dysplasia and X-Linked Hypophosphatemia in a Three-Generation Chinese Family
Calcified Tissue International Open Access 06 June 2023
-
Sequencing by avidity enables high accuracy with low reagent consumption
Nature Biotechnology Open Access 25 May 2023
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Sanger, F. et al. Nature 24, 687–695 (1977).
Sanger, F., Nicklen, S. & Coulson, A.R. Proc. Natl. Acad. Sci. USA 74, 5463–5467 (1977).
Maxam, A.M. & Gilbert, W. Proc. Natl. Acad. Sci. USA 74, 560–564 (1977).
Sanger, F. & Coulson, A.R. J. Mol. Biol. 94, 441–448 (1975).
Margulies, M. et al. Nature 437, 376–380 (2005).
Shendure, J. et al. Science 309, 1728–1732 (2005).
Edwards, R.A. et al. BMC Genomics 7, 57 (2006).
Sogin, M.L. et al. Proc. Natl. Acad. Sci. USA 103, 12115–12120 (2006).
Angly, F.E. et al. PLoS Biol. 4, 2121–2131 (2006).
Poinar, H.N. et al. Science 311, 392–394 (2006).
Green, R.E. et al. Nature 444, 330–336 (2006).
Noonan, J.P. et al. Science 314, 1113–1118 (2006).
Andries, K. et al. Science 307, 223–227 (2005).
Velicer, G.J. et al. Proc. Natl. Acad. Sci. USA 103, 8107–8112 (2006).
Goldberg, S.M. et al. Proc. Natl. Acad. Sci. USA 103, 11240–11245 (2006).
Gilbert, M.T.P. et al. Science 317, 1927–1930 (2007).
Cheung, F. et al. BMC Genomics 7, 272 (2006).
Ohtsu, K. et al. Plant J. 52, 391–404 (2007).
Thomas, R.K. et al. Nat. Genet. 39, 347–351 (2007).
Acknowledgements
I thank W. Miller and N. Wittekindt for suggestions on the manuscript. The author is funded in part by the Gordon and Betty Moore Foundation.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schuster, S. Next-generation sequencing transforms today's biology. Nat Methods 5, 16–18 (2008). https://doi.org/10.1038/nmeth1156
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth1156
This article is cited by
-
Highly Aligned Ternary Nanofiber Matrices Loaded with MXene Expedite Regeneration of Volumetric Muscle Loss
Nano-Micro Letters (2024)
-
Sequencing by avidity enables high accuracy with low reagent consumption
Nature Biotechnology (2024)
-
Screening and identification of potential key biomarkers for glucocorticoid-induced osteonecrosis of the femoral head
Journal of Orthopaedic Surgery and Research (2023)
-
Organ-specific expression of genes associated with the UDP-glucose metabolism in sugarcane (Saccharum spp. hybrids)
BMC Genomics (2023)
-
Regulation of omega-3 fatty acids production by different genes in freshwater fish species: a review
Fish Physiology and Biochemistry (2023)
