Abstract
The nucleotide sequence of the region between the spike (S) and the membrane (M) protein genes, and sequences of the S and ns2 genes of the OC43 strain of human coronavirus (HCV-OC43) were determined. The ns2 gene comprises an open reading frame (ORF) encoding a putative nonstructural (ns) protein of 279 amino acids with a predicted molecular mass of 32-kDa. The S gene comprises an ORF encoding a protein of 1353 amino acid residues, with a predicted molecular weight of 149,918. Sequence comparison between HCV-OC43 and the antigenically related bovine coronavirus (BCV) revealed more sequence divergence in the putative bulbous part of the S protein (S1) than in the stem region (S2). The cysteine residues near the transmembrane domain and the internal predicted protease cleavage site are conserved in the HCV-OC43 S protein. Nucleotide sequence analysis of the region between the S and M gene loci revealed the presence of an unexpected intragenomic partial leader sequence and two ORFs encoding potential proteins of 12.9 and 9.5-kDa. These two proteins were identified as nonstructural by comparison with the homologous BCV genes. In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, but is expressed in infected cells, as shown by immunofluorescence. Interestingly, two ORFs, potentially encoding 4.9 and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, indicating that they are not essential for viral replication in HRT-18 cells.
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Keywords
- Amino Acid Sequence Identity
- Nonstructural Protein
- Mouse Hepatitis Virus
- Polyclonal Rabbit Antiserum
- Spike Protein
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References
W. Spaan, D. Cavanagh, and M.C. Horzinek. In: “Immunochemistry of viruses, II. The basis for serodiagnosis and vaccines”. M.H.V. van Regenmortel and A.R. Neurath, eds., p. 359, Elsevier, New York (1990).
K.V. Holmes. In: “Virology, 2nd edn.”, B.N. Fields, D.M. Knipe et al., eds., p. 841, Raven Press, New York (1990).
H. Wege, S. Siddell, and V. ter Meulen. Curr. Top. Microbiol. Immunol. 99: 165 (1982).
M.M.C. Lai. Annu. Rev. Microbiol. 44: 303 (1990).
B.G. Hogue, B. King, and D.A. Brian. J. Virol. 51: 384 (1984).
B.G. Hogue, and D.A. Brian. Virus Res. 5: 131 (1986).
X.M. Zhang, K.G. Kousoulas, and J. Storz. Virology 186: 318 (1992).
S. Mounir, and P.J. Talbot. J. Gen. Virol. 73: 2731 (1992).
T. Kamahora, L.H. Soe, and M.M.C. Lai. Virus Res. 12: 1 (1989).
E. Southern. Methods Enzymol. 69: 152 (1979).
M. Cogné, S. Mounir, J.L. Preud’homme, F. Nau, and P. Guglielmi. Eur. J. Immunol. 18: 1485 (1988).
J.L. Leibowitz, S. Perlman, G. Weinstock, J.R. DeVries, C. Budzilowicz, J.M. Weissemann, and S.R. Weiss. Virology 164: 156 (1988).
S. Abraham, T.E. Kienzle, W.E. Lapps, and D.A. Brian. Virology 177: 488 (1990).
M. Kozak. J. Cell Biol. 108: 229 (1989).
G.J. Cox, M.D. Parker, and L.A. Babiuk. Nucl. Acids Res. 17: 5847 (1989).
S. Abraham, T.E. Kienzle, W. Lapps, and D.A. Brian. Virology 176: 296 (1990).
C.J. Budzilowicz, and S.R. Weiss. Virology 157: 509 (1987).
D.X. Liu, and S.C. Inglis. Virology 185: 911 (1991).
M. Godet, R. L’Haridon, J.-F Vautherot, and H. Laude. Virology 188: 666 (1992).
B. Schwarz, E. Routledge, and S.G. Siddell. J. Virol. 64: 4784 (1990).
K. Yokomori, and M.M.C. Lai. J. Virol. 65: 5605 (1991).
D. Rasschaert, M. Duarte, and H. Laude. J. Gen. Virol. 71: 2599 (1990).
T.M. Gallagher, S.E. Parker, and M.J. Buchmeier. J. Virol. 64: 731 (1990).
G. Gerna, N. Passarani, M. Battaglia, and E.G. Rondanelli. J. Inf. Dis. 151: 796 (1985).
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© 1994 Springer Science+Business Media New York
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Mounir, S., Labonté, P., Talbot, P.J. (1994). Characterization of the Nonstructural and Spike Proteins of the Human Respiratory Coronavirus OC43: Comparison with Bovine Enteric Coronavirus. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_10
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DOI: https://doi.org/10.1007/978-1-4615-2996-5_10
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